Zuordnung zu Einrichtungen:
- Inst. f. Mikrobiologie
- Naturwissenschaftliche Fakultät
Publikationen/Forschungsprojekte:
- Liste der Publikationen
Prof. Thomas Brüser studied Biology (Biochemistry, Genetics, Organic Chemistry) at the University of Cologne and the University of Sussex (Brighton, UK) from 1989 to 1994.
(NIH) 73-361 12 years ago in Hannover. He emphasized. 1 Univ.-Frauenklinik und Hebarnrnenschuie, losef-Schneider-Str. 4, D-8700 Wiirzburg. My present co-worker Herr Junge (1967), who was at that time studying. Timor-Tritsch IE, Dierker LJ, Zador I, Hertz RH, Rosen MG (1978) Fetal movements associated.
1995-1999: PhD studies at the University of Bonn
1999: PhD in Microbiology: “Enzymology of oxidative sulfur metabolism: Biochemistry and genetics of bacterial APS:phosphate adenylyl transferases”
2000-2001: Postdoc at the University of Pennsylvania (Philadelphia, USA): Cytochrome cbb3biogenesis in Rhodobacter capsulatus and Tat-dependent protein translocation in Escherichia coli
2002-2009: Research group leader at the University of Halle-Wittenberg
2006: Habilitation in Microbiology: “Tat-dependent transport of proteins across biological membranes”
Since Oct. 2009: Full professor (W3) at the Leibniz University Hannover
Awards
2000: PhD award of the German Society of General and Applied Microbiology (VAAM)
2007: Christian-Wolff-Award of the University of Halle-Wittenberg
Research interests
- Transport of folded proteins by the bacterial Tat pathway (more)
- Membrane stress and the phage shock response (more)
- Periplasmic pyoverdine maturation and modification
- Biofilm formation by plant growth-promoting rhizobacteria
- Protein folding and cofactor assembly pathways
- Bacterial chaperone systems
- Membrane protein complexes
- Microbial cell biology
- Anaerobic respirations
- Bacterial photosynthesis pathways
44. | Geise, H., Heidrich, E.-S., Nikolin, C.S., Mehner-Breitfeld, D., and Brüser, T. (2019) A potential late stage intermediate of twin-arginine dependent protein translocation in Escherichia coli. Front. Microbiol., 10:1482 |
43. | Mehner-Breitfeld, D., Rathmann, C., Riedel, T., Just, I., Gerhard, R., Overmann, J., and Brüser, T. (2018) Evidence for an adaptation of a phage-derived holin/endolysin system to toxin transport in Clostridioides difficile. Front. Microbiol., 9:2446 |
42. | Ringel, M.T., and Brüser, T. (2018) The biosynthesis of pyoverdines. Microb. Cell, 5, 424-437 |
41. | Heidrich, E.S., and Brüser, T. (2018) Evidence for a second regulatory binding site on PspF that is occupied by the C-terminal domain of PspA. PLoS ONE 13, e0198564 |
40. | Hou, B., Heidrich, E.S., Mehner-Breitfeld, D., and Brüser, T. (2018) The TatA component of the twin-arginine translocation sysem locally weakens the cytoplasmic membrane of Escherichia coli upon protein substrate binding. J. Biol. Chem.293, 7592-7605 |
39. | Ringel, M.T., Dräger, G., and Brüser, T. (2018) PvdO is required for the oxidation of dihydropyoverdine as last step of fluorophore formation in Pseudomonas fluorescens. J. Biol. Chem.293, 2330-2341 |
38. | Ringel, M.T., Dräger, G., and Brüser, T. (2017) The periplasmic transaminase PtaA of Pseudomonas fluorescens converts the glutamic acid residue at the pyoverdine fluorophore to α-ketoglutaric acid. J. Biol. Chem.292, 18660-18671 |
37. | Thurotte, A., Brüser, T., Mascher, T. and Schneider, D. (2017) Membrane chaperoning by members of the PspA/IM30 protein family, Commun Integr Biol. 10, e1264546 |
36. | Rathmann, C., Schlösser, A., Schiller, J., Bogdanov, M., and Brüser, T. (2017) Tat transport in Escherichia coli requires zwitterionic phosphatidylethanolamine but no specific negatively charged phospholipid. FEBS Lett. 591, 2848-2858 |
35. | Ringel, M.T., Dräger, G., and Brüser, T. (2016) PvdN enzyme catalyzes a periplasmic pyoverdine modification. J. Biol. Chem.291, 23929-23938 |
34. | Stolle, P., Hou, B., and Brüser, T. (2016) The Tat substrate CueO is transported in an incomplete folding state. J. Biol. Chem.291, 13520-13528 |
33. | Osadnik H, Schöpfel M, Heidrich E, Mehner D, Lilie H, Parthier C, Risselada HJ, Grubmüller H, Stubbs MT, Brüser T. (2015) The PspF-binding domain PspA1-144 and the PspA·F complex - New insights into the coiled-coil dependent regulation of AAA+ proteins. Mol. Microbiol.98, 743-759 |
32. | Taubert, J., Hou, B., Risselada, H.J., Mehner, D., Lünsdorf, H., Grubmüller, H., and Brüser, T. (2015) TatBC-independent TatA/Tat substrate interactions contribute to transport efficiency. PLoS ONE10, e0119761 |
31. | Taubert, J., and Brüser, T. (2014) Twin-arginine translocation-arresting protein regions contact TatA and TatB. Biol. Chem.395, 827-836 |
30. | Niggemann, J., Bozko, P., Bruns, N., Wodtke, A., Gieseler, M.T., Thomas, K., Jahns, C., Nimtz, M., Reupke, I., Brüser, T., Auling, G., Malek, N., Kalesse, M. (2014) Baceridin, a cyclic hexapeptide from an epiphytic Bacillus strain, inhibits the proteasome. Chembiochem. 15, 1021-1029 |
29. | Behrendt J, Brüser T. (2014) The TatBC complex of the Tat protein translocase in Escherichia coli and its transition to the substrate-bound TatABC complex. Biochemistry53, 2344-2354 |
28. | Mehner, D., Osadnik, H., Lünsdorf, H., and Brüser, T. (2012) The Tat system for membrane translocation of folded proteins recruits the membrane-stabilizing Psp machinery in Escherichia coli. J. Biol. Chem. 287, 27834-27842 |
27. | Brehmer, T., Kerth, A., Graubner, W., Malesevich, M., Hou, B., Brüser, T., and Blume, A. (2012) Negatively charged phospholipids trigger the interaction of a bacterial Tat substrate precursor with lipid monolayers. Langmuir28, 3534-3541 |
26. | Hou, B., and Brüser, T. (2011) The Tat-dependent protein translocation pathway. Biomol. Concepts 2, 507-523 |
25. | Lindenstrauß, U., Matos, C., Graubner, W., Robinson, C., and Brüser, T. (2010) Malfolded recombinant Tat substrates are Tat-independently degraded in Escherichia coli. FEBS Lett.584, 3644-3648 |
24. | Lindenstrauß, U., and Brüser, T. (2009) Tat-transport of linker-containing proteins in Escherichia coli. FEMS Lett.295, 135-140 |
23. | Weininger, U., Haupt, C., Schweimer, K., Graubner, W., Kovermann, M., Brüser, T., Scholz, C., Schaarschmidt, P., Zoldak, G., Schmidt, F.X., and Balbach, J. (2009) NMR solution structure of SlyD from Escherichia coli: Spatial separation of prolyl isomerase and chaperone function. J. Mol. Biol.387, 295-305 |
22. | Standar, K., Mehner, D., Osadnik, H., Berthelmann, F., Hause, G., Lünsdorf, H., and Brüser, T. (2008) PspA can form large scaffolds in Escherichia coli. FEBS Lett. 582, 3585-3589 |
21. | Berthelmann, F., Mehner, D., Richter, S., Lindenstrauß, U. Hause, G., Lünsdorf, H., and Brüser, T. (2008) Recombinant expression of tatABC and tatAC results in the formation of interacting cytoplasmic TatA-tubes in Escherichia coli. J. Biol. Chem.283, 25281-25289 |
20. | Natale, P., Brüser, T., and Driessen, A.J. (2008) Sec- and Tat-mediated protein secretion across the bacterial cytoplasmic membrane - distinct translocases and mechanisms. BBA Biomembranes1778, 1735-1756 |
19. | Richter, S., Lindenstrauß, U., Lücke, C., Bayliss, R., and Brüser, T. (2007) Functional Tat transport of unstructured, small, hydrophilic proteins. J. Biol. Chem.282, 33257-33264 |
18. | Behrendt, J., Lindenstrauß, U., and Brüser, T. (2007) TatC recruits TatB and prevents its multimerization in Escherichia coli. FEBS Lett. 581, 4085-4090 |
17. | Brüser, T. (2007) The twin-arginine translocation system and its capability for protein secretion in biotechnological protein production. Appl. Microbiol. Biotechnol.76, 35-45 |
16. | Graubner, W., Schierhorn, A., and Brüser, T. (2007) DnaK plays a pivotal role in Tat targeting of CueO and functions beside SlyD as a general Tat signal binding chaperone. J. Biol. Chem.282, 7116-7124 |
15. | Lindenstrauss, U., and Brüser, T. (2006) Conservation and variation between Rhodobacter capsulatus and Escherichia coli Tat systems. J. Bacteriol.188, 7807-7814 |
14. | Sturm, A., Schierhorn, A., Lindenstrauss, U., Lilie, H., and Brüser, T. (2006) YcdB from Escherichia coli reveals a novel class of Tat-dependently translocated hemoproteins. J. Biol. Chem., 281, 13972-13978 |
13. | Richter, S., and Brüser, T. (2005) Targeting of unfolded PhoA to the Tat translocon of Escherichia coli. J. Biol. Chem.280, 42723-42730 |
12. | Berthelmann, F., and Brüser, T. (2004) Localization of the Tat translocon components in Escherichia coli. FEBS Lett. 569, 82-88 |
11. | Behrendt, J., Standar, K., Lindenstrauss, U., and Brüser, T. (2004) Topological studies on the twin-arginine translocase component TatC. FEMS Microbiol. Lett. 234, 303-308 |
10. | Kipping, M., Lilie, H., Lindenstrauss, U., Andreesen, J.R., Griesinger, C., Carlomagno, T., and Brüser, T. (2003) Structural studies on a twin-arginine signal sequence. FEBS Lett. 550, 18-22 |
9. | Brüser, T., Brune, D., Yano, T., and Daldal, F. (2003) Membrane targeting of a folded and cofactor containing protein. Eur. J. Biochem.270, 1211-1221 |
8. | Brüser, T., and Sanders, C. (2003) An alternative model of the twin-arginine translocation system, Microbiol. Res. 158, 7-17 |
7. | Rose, R. W., Brüser, T., Kissinger, J.C., and Pohlschröder, M. (2002) Adaptation of protein secretion to extremely high salt conditions by extensive use of the twin arginine translocation pathway. Mol. Microbiol.45, 943-950 |
6. | Brüser, T., Selmer, T., and Dahl, C. (2000) 'ADP sulfurylase' from Thiobacillusdenitrificans is an adenylylsulfate:phosphate adenylyltransferase and belongs to a new family of nucleotidyltransferases. J. Biol. Chem. 275, 1691-1698 |
5. | Brüser, T., Deutzmann, R., and Dahl, C. (1998) Evidence against the double-arginine motif as the only determinant for protein translocation by a novel Sec-independent pathway in Escherichia coli. FEMS Microbiol. Lett.164, 329-336 |
4. | Reinartz, M., Tschäpe, J., Brüser, T., Trüper, H.G., and Dahl, C. (1998) Sulfide oxidation in the phototrophic sulfur bacterium Chromatium vinosum. Arch. Microbiol. 170, 59-68 |
3. | Brüser, T., Trüper, H.G., and Dahl, C. (1997) Cloning and sequencing of the gene encoding the high potential iron-sulfur protein (HiPIP) from the purple sulfur bacterium Chromatium vinosum. Biochim. Biophys. Acta1352, 18-22 |
2. | Strange, R.W., Dodd, F.E., Abraham, Z.H.L., Grossmann, J.G., Brüser, T., Eady, R.R., Smith, B.E., and Hasnain, S.S. (1995) The substrate binding site in nitrite reductase and its similarity to Zn carbonic anhydrase. Nature Struct. Biol.2, 287-292 |
1. | Howes, B.D., Abraham, Z.H.L., Lowe, D.J., Brüser, T., Eady, R.R., and Smith, B.E. (1994) EPR and electron nuclear double resonance (ENDOR) studies show nitrite binding to type two copper centers of the dissimilatory nitrite reductase of Alcaligenes xylosoxidans (NCIMB 11015). Biochemistry33, 3171-3177 |